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Homologous Recombination

RNAi off-Target Effects

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Published Data

An analysis of normalization methods for Drosophila RNAi genomic screens and development of a robust validation scheme (submitted)
Wiles, A.M, Ravi, D., Bhavani, S., Bishop, A.J.R

- raw_data.txt
Contains raw data from untreated control and MMS screening plates for each DRSC amplicon tested for replicates 1 through 4 (control) and 1 through 3 (MMS).

- normalization_data.txt
Contains normalized data values and rank for each method, along with whether the amplicon validated as being required after MMS treatment (validation_hit = 1) or not (validation_hit = 0). Normalizations reported: background subtraction (bs), background corrected (bc), quantile normalization (qn), background subtraction followed by quantile normalization (bsqn), background correction followed by quantile normalization (bcqn), interquartile range measurement (iqr), and cellHTS2.

Interactome Paper - Data