Are there any special instructions for Optical Imaging?
One of the above anesthetizing methods can be used for optical imaging. Common modes of scanning are luminescence and fluorescence. Due to light scattering and absorption property of the tissue, the signal tends to be very weak. To maximize the signal, it is recommended to shave the area of interest before scanning. Commercial clippers or a beard trimmer can be used for this purpose. In many cases, the signal from the animal’s abdomen seems to give better information than from its back, so we recommend that the animal be laid with its belly up for optical scans. D-Luciferin required for imaging luciferase reporter animals is available through Caliper Life Sciences.
Are there any special instructions for microUltrasound?
Anesthetize the animal using the above method before scanning. For ultrasound imaging we recommend that the hair of animal overlying the region of interest be removed completely. This can be done using clippers followed by a commercially available hair removal solution (Nair). Ultrasound waves do not travel well when there is air between the probe and the skin, so an ultrasound gel is used as an interface medium.
Does the animal require temperature control?
It is imperative to maintain optimum temperature for the animal following anesthesia to prevent hypothermic demise. We suggest using a heat pad with a control to vary the heat settings. To ensure even warming, place a glass sheet in-between the heat pad and the animal.
Do we need gas exchange filters to prevent transferring infections between our animals and the Imaging Facility?
To prevent infection to the animal and the facility, small particle vacuum filters can be used along with an air-tight chamber. We recommend the Vacu-Guard filter available from Cole-Palmer
Do the animals need to be shaved before imaging?
As noted above for Optical Imaging, it is recommended to shave the animal before scanning. Commercially available clippers can be used for this purpose.
Do the animals need to be "naired" before imaging?
"Nairing" is necessary only for microUltrasound imaging. This can be done using a commercially available hair removal solution. We recommend only limited use of Nair in conjunction with adequate warming during hair removal.
Are special diets necessary?
Normal diets for mice and rats have materials that interfere with the scanning procedures (microCT, Luminescence, and Fluorescence). So we recommend use of special diets at least 3 days before scanning. We have tested the Teklad Diet TD.05109 and it works very well to eliminate unwanted signal from intestines and bladder for MicroCT and Optical Imaging.
What procedures should be followed before we bring animals to your facility?
For imaging live animals, they should be brought into the facility through the docking area of GCCRI. The animals will be taken into the facility in an air-tight chamber with filters attached. It is the responsibility of the customer to anesthetize the animal and place them in a special chamber that we provide. The special filters can be brought from a vendor or GCCRI can provide them for a charge. After the imaging procedure the animals will be returned immediately.
What procedures should be followed to send non-live specimens?
For imaging non-live specimens they should be packed inside a plastic tube or jar of not more than 3” diameter, and sealed with parafilm. We prefer that you make the specimen immobile while it is in the tube by using packing foam, and mark the region to be scanned on the outside. The specimen can be suspended in 100% ethanol. If you want to use any other solution, please contact the facility manager.
What procedures should be followed to send non-live specimens?
Absolutely! Just contact Mr. Pablo Gonzales from LAR at 567-0564 to arrange the transfer.