The Genome Sequencing Facility (GSF) is currently located in the 2nd-floor laboratory space (Rm. 2.120) in Greehey Children’s Cancer Research Institute building. It has approximately 1000 sq. ft. of research space, which contains bench stations and a set of standard equipment for a fully operational molecular biology lab. There are dedicated spaces for sample QC, library preparation and QC, cBot amplification and set up the sequencing run.
The Genome Sequencing Facility (GSF) is equipped with the most popular Illumina NGS portfolio including Illumina HiSeq 3000 system, Illumina NextSeq 500, and Illumina MiSeq system for the high throughput next-generation sequencing, and high-performance computer cluster for massive data processing and storage requirements. The genomics laboratory is equipped with specialized equipment needed to create the range of sequencing libraries from genomic DNA, ChIP-DNA, RNA and small RNA samples.
- Illumina HiSeq 3000 Sequencing System
- Illumina NextSeq 500 Sequencer
- Illumina MiSeq Sequencer
- Illumina cBot Cluster Generation Station
- Covaris S220 Ultra Sonicator
- Beckman Coulter SPRIworks Fragment Library System I for Illumina
- Agilent 2100 Bioanalyzer
- Advanced Analytical Fragment Analyzer
- Invitrogen Qubit 2.0 & 3.0 Fluorometer
- Eppendorf Plate Centrifuge
- SpeedVac Centrifuge
- Eppendorf Realplex Quantitative PCR
- Thermo Scientific NanoDrop 2000
- Eppendorf Thermo-Cyclers
Illumina cBot Cluster generation system, MiSeq, NextSeq 500 and HiSeq 3000 sequencing system are highly specialized equipment, only the trained and qualified people can have direct access to the sequencing instrumentation. The GSF manages a Covaris sonicator and Agilent Bioanalyzer which are available for NGS users. These instruments are critical to many NGS processes and are frequently used by the GSF. To balance these demands, we have established user service mechanism to use these instruments: users are trained to use instruments and they are responsible for any reagents/consumables used for the instruments. There is no instrumentation usage charge if they process the samples that will be used for sequencing at GSF. Otherwise, there is associated instrumentation usage charge if they process the samples not related to the GSF.
Attn: Dawn Garcia/Zhao Lai
Greehey Children’s Cancer Research Institute, Room 2.120
UT Health San Antonio
8210 Floyd Curl Drive
San Antonio, Texas 78229