Pricing

The fee schedule was formulated on the basis of operating expenses of the laboratory in addition to comparison with other academic NGS facilities. For some multi-faceted complex investigations, a project charge is agreed upon to cover a specific time period and estimated number of analyses. Fees are reviewed annually and adjusted as necessary, with guidance from the Science Advisory Committee. The GSF offers the significant discount for large sample size projects. The discount rate varies case by case, depending on the sample size. Please contact with Director Dr. Zhao Lai for details.

NGS HiSeq3000 Sequencing/lane

Sequencing Type

Internal

External

Major Applications

50bp Single Read Sequencing

(1 x 50 cycles)

$1,000 $1,100 RNA-seq, ChIP-seq

75bp Paired End Sequencing

(2 x 75 cycles)

$1,850 $1,950 RNA-seq, exome seq for DNA varients

100bp Paired End Sequencing

(2 x 100 cycles)

$2,260 $2,360 RNA-seq for splicing varients, exome seq for DNA varients

150bp Paired End Sequencing

(2 x 150 cycles)

$2,550 $2,650 Whole genome sequencing

NGS NextSeq 500 Sequencing/run

Sequencing Type

Internal

External

Major Applications

Mid 75bp PE $1,300 $1,450 RNA
Mid 150bp PE $2,050 $2,250 Whole Genome
High 75bp SR $1,750 $1,900 RNA-, SmallRNA
High 75bp PE $3,200 $3,600 RNA-, Exome-seq
High 150bp PE $4,800 $5,500 Whole Genome

NGS MiSeq Sequencing/run

Sequencing Type

Internal

External

Major Applications

75bp Paired End Sequencing

(2 x 75 cycles)

$1,100 $1,100 Targeted gene re-sequencing, Targeted RNA expression

300bp Paired End Sequencing

(2 x300 cycles)

$1,800 $1,900 16S metagenomicss

Illumina Library Preparation

(Includes Sample QC, library preparation, normalization and pooling with Index)

Library Type

Internal

Outside

DNA-Seq (with Nextera XT for small genomes < 5Mb) $80 $110
DNA-Seq $100 $120
mRNA-Seq (with PolyA selection) $140 $170
Total RNA-Seq (with rRNA depletion) $160 $190
ChIP-Seq $120 $170
Small RNA-Seq (with TruSeq small RNA kit) $180 $210
Small RNA-Seq (with NEB or TriLink small RNA kit) $140 $160
Nextera Rapid Exome Capture Enrichment $200 $230
TruSeq Exome Capture Enrichment $200 $230
16S Metagenomics sequencing per reaction $30 $40

Sample QC, library QC

Sample Type

Rate

NGS library QC

(for up to 11 samples)

$5

RNA sample QC

for up to 12 samples for RNA nano chip)

$85

RNA sample QC

(for up to 11 samples for RNA pico chip)

$85

Bioinformatics analysis

Object

Internal

Outside

RNA-seq

(including smRNA-seq)

$100 $120
DNA-seq $100 $120