Research and Collaboration Service

The Genome Sequencing Facility (GSF) provides complete project consultation for optimal experimental design and set up. The GSF is also a research group with experience in customizing experiments and developing new protocols that leverage the latest advances in genomic technology.

The GSF performs all protocols necessary for preparing a biological sample to be sequenced on the Illumina HiSeq 3000 system, Illumina NextSeq 500 and Illumina MiSeq. For next-generation sequencing studies, the GSF provides the library preparation and sequencing for the following applications:

  • DNA seq (whole genome de novo sequencing, whole exome sequencing, candidate gene re-sequencing, target sequencing, amplicon sequencing)
  • RNA seq (Total RNA sequencing, stranded mRNA sequencing, RIP sequencing, CLIP sequencing)
  • Small RNA seq
  • ChIP seq
  • MBDCap DNA seq
  • Single cell DNA & RNA seq
  • 16S based metagenomics
  • Targeted gene re-sequencing
  • Targeted gene expression
  • Cancer gene panel

The GSF performs single cell analysis through 10X Genomics Chromium system which acquired at July of 2019.   For 10X Genomics single cell studies, the GSF provides the library preparation and sequencing for the following applications:

  • Single cell RNA-seq: Provide 3’ digital gene expression by profiling 500 to 10,000 individual cells per sample
  • Single cell ATAC-seq: Determine the regulatory landscape of chromatin to study epigenomics
  • Single cell Immune Profiling: Sequence VDJ and TCR regions while assessing the gene expression profiles of immune cells
  •  More applications will follow from the updates of 10X Genomics new products

Bioinformatics analysis is available through CBBI (Computational Biology and Bioinformatics Initiative) led by Dr. Yidong Chen.