CRISPR Libraries List


Control vectors (Addgene)

pAW12.lentiguide.GFP (item #104374)
LentiCRISPRv2GFP (item #82416) 

Pooled CRISPR Libraries (Addgene)

Human sgRNA library Brunello in lentiCRISPRv2 (item #73179) 

https://www.addgene.org/search/catalog/pooled-libraries/?q=item+%2373179

Human CRISPR activation pooled library (Calabrese 65 HSF, Set A, item #92379)
Human CRISPR activation pooled library (Calabrese 65 HSF, Set B, item #92380)

https://www.addgene.org/pooled-library/broadgpp-human-crispra-calabrese-p65hsf/)

Human CRISPR inhibition pooled library (Dolcetto in backbone XPR_050, Set A, item #92385)
Human CRISPR inhibition pooled library (Dolcetto in backbone XPR_050, Set B, item #92386)

https://www.addgene.org/pooled-library/broadgpp-human-crispri-dolcetto/

Arrayed CRISPR Libraries (Synthego)

Human whole genome pooled CRISPR knockout library

Synthego’s CRISPR Screening Libraries are collections of synthetic sgRNAs in arrayed multiwell plates. When combined with SpCas9, each library targets and knocks out a range of human genes in a biologically relevant pathway. Each multiwell plate consists of either a standard (pre-defined) or custom library with multiplexed guide design (i.e., up to 3 modified synthetic sgRNA guides targeting each gene).

Product Specifics

  • Libraries have a strategic multiplex design: up to 3 modified synthetic sgRNA guides targeting one gene per well.
  • Multiplexed sgRNAs are designed to induce fragment deletions (21+ bp) in each gene, increasing the likelihood of complete loss of function.
  • All sgRNAs are chemically modified to resist degradation ( 2′ O-methyl modifications and 3’ phosphorothioate bonds in the first and last three terminal bases).
  • The guides are designed to target an early exon that is present in as many transcripts as possible. Ninety percent of multiplex guides target the first 1/3 of the protein-coding region, and all constructs target exons that are found in >75% of known variants.
  • All guides are bioinformatically screened for off-target matches. Ninety-six of the whole human genome (19,753 genes) have stringent guide designs that prohibit exact matches, and off-by-one bp matches to minimize off-target effects.