Molecular Cell: SRSF2 Plays an Unexpected Role as Reader of m5C on mRNA, Linking Epitranscriptomics to Cancer (Gupta)

December 7, 2023

 Hai-Li Ma 1 14, Martin Bizet 1 14, Christelle Soares Da Costa 1 14, Frédéric Murisier 1, Eric James de Bony 1 8, Meng-Ke Wang 2, Akihide Yoshimi 3 9, Kuan-Ting Lin 7, Kristin M. Riching 4, Xing Wang 2, John I. Beckman 5, Shailee Arya 5, Nathalie Droin 6, Emilie Calonne 1, Bouchra Hassabi 1, Qing-Yang Zhang 2 10, Ang Li 2 11, Pascale Putmans 1, Lionel Malbec 1, Céline Hubert 1…François Fuks 1 15 Highlights SRSF2 preferentially binds m5C-marked mRNA, whereas SRSF2P95H mutant impairs binding NSUN2 depletion reduces mRNA m5C levels and alters SRSF2 RNA binding and splicing NSUN2 loss and SRSF2P95H alter SRSF2 binding to key leukemia-related transcripts In leukemia patients, low NSUN2 levels and SRSF2P95H mutation predict poor outcomes. Summary A common mRNA [...]

Nucleic Acids Research: TATDN2 resolution of R-loops is required for survival of BRCA1-mutant cancer cells (Sung, Gupta, Pertsemlidis, Bishop, et al)

November 29, 2023

 Aruna S Jaiswal, Arijit Dutta, Gayathri Srinivasan, Yaxia Yuan, Daohong Zhou, Montaser Shaheen, Doraid T Sadideen, Austin Kirby, Elizabeth A Williamson, Yogesh K Gupta, Shaun K Olsen, Mingjiang Xu, Eva Loranc, Pramiti Mukhopadhyay, Alexander Pertsemlidis, Alexander J R Bishop, Patrick Sung, Jac A Nickoloff, Robert Hromas BRCA1-deficient cells have increased IRE1 RNase, which degrades multiple microRNAs. Reconstituting expression of one of these, miR-4638–5p, resulted in synthetic lethality in BRCA1-deficient cancer cells. We found that miR-4638-5p represses expression of [...]

Nucleic Acids Research: TATDN2 resolution of R-loops is required for survival of BRCA1-mutant cancer cells (Gupta, Pertsemlidis, Bishop, & Sung Labs)

November 20, 2023

 Aruna S Jaiswal, Arijit Dutta, Gayathri Srinivasan, Yaxia Yuan, Daohong Zhou, Montaser Shaheen, Doraid T Sadideen, Austin Kirby, Elizabeth A Williamson, Yogesh K Gupta, Shaun K Olsen, Mingjiang Xu, Eva Loranc, Pramiti Mukhopadhyay, Alexander Pertsemlidis, Alexander J R Bishop, Patrick Sung, Jac A Nickoloff, Robert Hromas Abstract BRCA1-deficient cells have increased IRE1 RNase, which degrades multiple microRNAs. Reconstituting expression of one of these, miR-4638–5p, resulted in synthetic lethality in BRCA1-deficient cancer cells. We found that miR-4638-5p represses [...]

Dr. Gupta to Present at Cold Spring Harbor Laboratory Meeting on Eukaryotic mRNA Processing, August 22-26, 2023

August 2, 2023

 Click on image below for full agenda _________________________________________________________ Since 2004, UT Health San Antonio, Greehey Children’s Cancer Research Institute’s (Greehey CCRI) mission has been to advance scientific knowledge relevant to childhood cancer, contribute to understanding its causes, and accelerate the translation of knowledge into novel therapies. Greehey CCRI strives to have a national and global impact on [...]

Midwest AViDD Center announces funding for six high-potential projects to further antiviral drug discovery (Gupta Lab et al)

July 10, 2023

 MINNEAPOLIS/ST. PAUL (07/05/2023) – To accelerate the Midwest Antiviral Drug Discovery (AViDD) Center's mission to develop therapeutics for viruses with pandemic potential, the Center today announced $3 million in funding for six additional projects over the next two years. The projects will expand the Center's expertise across disciplines to discover effective responses to pandemics, life-threatening infections, [...]

Cancer Research: M6A RNA Methylation Regulates Histone Ubiquitination to Support Cancer Growth and Progression (Gupta, Zheng, Houghton, Chen, and Rao Labs)

March 21, 2022

 Pooja Yadav; Panneerdoss Subbarayalu; Daisy Medina, Saif Nirzhor;  Santosh Timilsina; Subapriya Rajamanickam; Vijay K. Eedunuri; Yogesh Gupta Siyuan Zheng Nourhan Abdelfattah Yufei Huang Ratna Vadlamudi Robert Hromas; Paul Meltzer Peter Houghton; Yidong Chen Manjeet K. Rao Abstract Osteosarcoma is the most common malignancy of the bone, yet the survival for osteosarcoma patients is virtually unchanged [...]

eLife: RNA binding to human METTL3-METTL14 restricts N6-deoxyadenosine methylation of DNA in vitro (Gupta Lab)

January 21, 2022

 Shan Qi,  Javier Mota,  Siu-Hong Chan,  Johanna Villarreal,  Nan Dai,  Shailee Arya,  Robert A Hromas,  Manjeet K Rao,  Ivan R Corrêa Jr  Yogesh K Gupta  Abstract Methyltransferase like-3 (METTL3) and METTL14 complex transfer a methyl group from S-adenosyl-L-methionine to N6 amino group of adenosine bases in RNA (m6A) and DNA (m6dA). Emerging evidence highlights the role of [...]