CRISPR Screening
CRISPR screening can yield powerful and robust data to find new genes and pathways, as well as give insights into the mechanisms of action of drugs, identify synthetic lethal partners. CRISPR/Cas9 screening can be divided into pooled screening and array-based screening. The pooled screening introduces a library of single guide RNAs (sgRNAs) that target different genes in cells, followed by applying a selection pressure (such as drug treatment) to enrich or deplete cells with specific sgRNA-mediated gene knockouts. In arrayed CRISPR screenings, each sgRNA is delivered separately to a distinct population of cells through transfection or electroporation. The cells are then cultured in individual wells of a multi-well plate and subjected to various assays to measure the phenotypes induced by the selection pressure (such as drug treatment). Our core facility provide both pooled and array-based genome-wide and sublibrary screening. We have the following libraries available for use:
- Human CRISPR Activation Pooled Library (Calabrese P65-HSF)
- Human CRISPR Inhibition Pooled library (Calabrese P65-HSF)
- Human CRISPR Knockout Pooled Library (Brunello) (pooled)
- Human Whole Genome Arrayed Library (Synthego)
siRNA Screening
TIF also provides genome-wide and sublibrary siRNA screening. The Human Genome siRNA Library (V4) contains 64,752 siRNA to cover 21,584 human genes. We also have the following defined human siRNA subsets for screening:
- Epigenetics
- Drug Targets
- DNA Damage
- Oncogenes
- Tumor Suppressors
- Cell Surface Receptors
- Kinases
- Phosphatases
- Membrane Trafficking
- Ubiquitinylation
- Ion Channels
- Nuclear Hormone Receptors
- Proteases
- Transcription Factors
miRNA Screening
- We also have the mirVana miRNA Mimic Library (2555 targets, mirBase 20) and mirVana miRNA inhibitor Library (2555 targets, mirBase 20) to assist customers in determining microRNA participation in biological processes such as development and growth, metabolic pathways and illnesses, and cancer progression.
Gene Editing and Isogenic Cell Generation
- We provide CRISPR/Cas9-based gene editing services to help researchers better understand the functions of genes in biological processes and develop assays for high throughput screening for drug discovery by performing gene knock-out, tag-knock-in, and isogenic cell generation.
High-throughput cell viability testing of single drug and drug combinations
- We assist customers in testing single drug or drug combinations in multiple cell lines in a high-throughput way.
Microscale Thermophoresis (MST) Assay Using Nanotemper
- MST is a biophysical assay that can be utilized to identify protein-protein, protein-DNA, and protein-drug interactions.
Activity-based protein profiling (ABPP) and proximity labeling (PL)
- We provide ABPP and PL for target deconvolution. Please contact Dr. Dongwen Lyu to discuss your needs for these assays.