Nature Methods: Sensitive and specific method for detecting G protein–coupled receptor mRNAs

Arne HansenYidong ChenJason M InmanQuang N PhanZhi-Qing QiCharlie C XiangMiklos PalkovitsNatasha ChermanSergei A KuznetsovPamela G RobeyEva Mezey & Michael J Brownstein

Abstract

G protein-coupled receptors (GPCRs) mediate the effects of extracellular signaling molecules in all the body’s cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

Learn More Button

Article Categories: All News, Featured news, Research Paper

Since 2004, UT Health San Antonio, Greehey Children’s Cancer Research Institute’s (Greehey CCRI) mission has been to advance scientific knowledge relevant to childhood cancer, contribute to understanding its causes, and accelerate the translation of knowledge into novel therapies. Greehey CCRI strives to have a national and global impact on childhood cancer by discovering, developing, and disseminating new scientific knowledge. Our mission consists of three key areas — research, clinical, and education.

Stay connected with the Greehey CCRI on Facebook, Twitter, LinkedIn, and Instagram.