Nature Methods: Sensitive and specific method for detecting G protein–coupled receptor mRNAs

Arne HansenYidong ChenJason M InmanQuang N PhanZhi-Qing QiCharlie C XiangMiklos PalkovitsNatasha ChermanSergei A KuznetsovPamela G RobeyEva Mezey & Michael J Brownstein

Abstract

G protein-coupled receptors (GPCRs) mediate the effects of extracellular signaling molecules in all the body’s cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

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