DNA Repair Protocols: Identification of Genes Required for Damage Survival Using a Cell-Based RNAi Screen Against the Drosophila Genome
Abstract
Exposure to DNA-damaging agents invokes biological responses necessary for damage recovery and cell survival. Despite the presence of intact DNA repair pathways, a lack of certain other biological pathways has been shown to sensitize cells to DNA-damaging agents’ exposure. It is likely that following DNA damage a complex interplay between DNA repair pathways and other biological pathways might be required to ensure cell survival. In this chapter, we describe a high-throughput method for the identification of genes essential for cell survival following DNA damage by using a cell-based assay to measure viability in combination with an RNA interference-based genome-wide screening experiment.
Keywords
DNA damage RNAi Genome-wide screening Drosophila Survival
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