The New Phytologist: Transcriptome divergence between introduced and native populations of Canada thistle, Cirsium arvense

Introduced plants may quickly evolve new adaptive traits upon their introduction. Canada thistle (Cirsium arvense – Cardueae, Asteraceae) is one of the worst invasive weeds worldwide. The goal of this study is to compare gene expression profiles of native (European) and introduced (North American) populations of this species, to elucidate the genetic mechanisms that may underlie such rapid adaptation. We explored the transcriptome of ten populations (five per range) of C. arvense in response to three treatments (control, nutrient deficiency, and shading) using a customized microarray chip containing 63 690 expressed sequence tags (ESTs), and verified the expression level of 13 loci through real-time quantitative PCR. Only 2116 ESTs (3.5%) were found to be differentially expressed between the ranges, and 4458 ESTs (7.1%) exhibited a significant treatment-by-range effect. Among them was an overrepresentation of loci involved in stimulus and stress responses. Cirsium arvense has evolved different life-history strategies on each continent. The two ranges notably differ with regard to R-protein mediated defense, sensitivity to abiotic stresses, and developmental timing. The fact that genotypes from the Midwest exhibit different expression kinetics than remaining North American samples further corroborates the hypothesis that the New World has been colonized twice, independently.