Genes & Cancer: Microarray profile of human kidney from diabetes, renal cell carcinoma and renal cell carcinoma with diabetes
Adam Kosti2, Hung-I Harry Chen4, Sumathy Mohan3, Sitai Liang2, Yidong Chen4,5, and Samy L. Habib1,2
ABSTRACT
A recent study from our laboratory showed that patients with diabetes are at a higher risk of developing kidney cancer. In the current study, we have screened the whole human DNA genome from healthy control, patients with diabetes or renal cell carcinoma (RCC), or RCC+diabetes. We found that 883 genes gain/163 genes loss of copy number in RCC+diabetes group, 669 genes gain/307 genes loss in RCC group, and 458 genes gain/38 genes loss of copy number in the diabetes group, after removing gain/loss genes obtained from the healthy control group. Data analyzed for functional annotation enrichment pathways showed that the control group had the highest number (280) of enriched pathways, 191 in the diabetes+RCC group, 148 in the RCC group, and 81 in the diabetes group. The overlap GO pathways between RCC+diabetes and RCC groups showed that nine were enriched, between RCC+diabetes and diabetes groups was four and between diabetes and RCC groups was eight GO pathways. Overall, we observed the majority of DNA alterations in patients from the RCC+diabetes group. Interestingly, insulin receptor (INSR) is highly expressed and had gains in copy number in RCC+diabetes and diabetes groups. The changes in the INSR copy number may use as a biomarker for predicting RCC development in diabetic patients.
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