Journal of Trauma & Acute Care Surgery: Whole Blood Resuscitation Restores Intestinal Perfusion and Influences Gut Microbiome Diversity (Lai)
Yracheta, Jaclyn MD1; Muraoka, Wayne PhD2; Wu, Xiaowu MD2; Burmeister, David PhD3; Darlington, Daniel PhD2; Zhao, David MD2; Lai, Zhao PhD4; Scroggins, Shannon MS1; Cap, Andrew P. MD, PhD2; Bynum, James PhD2; Nicholson, Susannah E. MD1,2
Abstract
Objective
Gut dysbiosis, an imbalance in the gut microbiome, occurs after trauma which may be ameliorated with transfusion. We hypothesized that gut hypoperfusion following trauma causes dysbiosis and that whole blood (WB) resuscitation mitigates these effects.
Methods
Anesthetized rats underwent sham (S; laparotomy only, n = 6), polytrauma (T; laparotomy, liver, and skeletal muscle crush injuries and femur fracture, n = 5), polytrauma and 40% hemorrhage (H; n = 7) and polytrauma, hemorrhage and WB resuscitation (R; n = 7) which was given as 20% estimated blood volume from donor rats 1 hr post-trauma. Baseline cecal mesenteric tissue oxygen (O2) concentration was measured following laparotomy and at 1- and 2 hrs post-trauma. Fecal samples were collected pre-injury and at euthanasia (2 hrs). 16 s rRNA sequencing was performed on purified DNA, and diversity and phylogeny were analyzed with QIIME using the Greengenes 16S rRNA database (OTUs; 97% similarity). Alpha- and β-diversity were estimated using observed species metrics. Permutational analysis of variance was performed for overall significance.
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Since 2004, UT Health San Antonio, Greehey Children’s Cancer Research Institute’s (Greehey CCRI) mission has been to advance scientific knowledge relevant to childhood cancer, contribute to the understanding of its causes, and accelerate the translation of knowledge into novel therapies. Through discovery, development, and dissemination of new scientific knowledge, Greehey CCRI strives to have a national and global impact on childhood cancer. Our mission consists of three key areas — research, clinical, and education.
Stay connected with the Greehey CCRI on Facebook, Twitter, LinkedIn, and Instagram.
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Since 2004, UT Health San Antonio, Greehey Children’s Cancer Research Institute’s (Greehey CCRI) mission has been to advance scientific knowledge relevant to childhood cancer, contribute to understanding its causes, and accelerate the translation of knowledge into novel therapies. Greehey CCRI strives to have a national and global impact on childhood cancer by discovering, developing, and disseminating new scientific knowledge. Our mission consists of three key areas — research, clinical, and education.
Stay connected with the Greehey CCRI on Facebook, Twitter, LinkedIn, and Instagram.