The Genome Sequencing Facility (GSF) at the Greehey Children’s Cancer Research Institute (GCCRI), serving as the Next Generation Sequencing Shared Resource (NGSSR) of the Mays Cancer Center, is pleased to invite you to a Spatial Transcriptomics Sample Preparation Seminar hosted in collaboration with 10x Genomics.
Spatial transcriptomics (ST) enables measurement of gene expression within the precise spatial context of tissue architecture. By preserving spatial information, ST provides powerful insight into cellular heterogeneity, cell–cell interactions, molecular gradients, and gene signatures associated with pathological features.
Current ST technologies generally fall into two categories:
- Sequencing-based ST (sST), such as Visium HD, where tissue sections are placed on spatially barcoded substrates. Transcripts are captured, sequenced, and computationally mapped back to their original locations to generate whole-transcriptome spatial maps.
- Imaging-based ST (iST), such as Xenium, which uses multiplexed fluorescence in situ hybridization (FISH) approaches to visualize and identify transcripts directly in tissue with single-molecule resolution across iterative imaging cycles.
These complementary platforms often serve different experimental goals. Visium HD is commonly used as a discovery tool due to its whole-transcriptome coverage, while Xenium is well suited for hypothesis-driven studies when specific targets or pathways—such as those in the tumor microenvironment—are already defined.
A critical determinant of success in any spatial transcriptomics project is high-quality tissue slide preparation. Recognizing this challenge, we have organized this seminar to provide practical guidance and support for optimal sample preparation.
During the seminar, Dr. Micah Castillo from 10x Genomics will present best practices for tissue slide preparation for both Visium HD and Xenium platforms. This session offers a valuable opportunity to learn how to effectively incorporate advanced spatial transcriptomics technologies into your research.
We warmly encourage investigators, postdoctoral fellows, graduate students, and research staff to attend. Please click the attache flyer to register seminar.