Neuro-Oncology: Dual Inhibition of MAPK and TORC1 Signaling Retards Development of Radiation Resistance in Pediatric BRAF V600E Glioma Models (Chen, Houghton, et al labs)

April 11, 2025

 Abstract Background MAPK pathway inhibitors (MAPKi) have shown significant efficacy in treating childhood BRAF-activated brain tumors. For tumors harboring BRAFV600E mutations, the drugs are rarely curative, and patients can become refractory to treatment. MAPKi, combining X-radiation therapy (XRT), may improve the cure rate, but the development of XRT-resistance is a challenge. Read Full Text

Cell: Fine-tuning of gene expression through the Mettl3-Mettl14-Dnmt1 axis controls ESC differentiation (Gupta Lab)

January 17, 2025

 Giuseppe Quarto1,12 ∙ Andrea Li Greci1,12 ∙ Martin Bizet1,12 ∙ Audrey Penning1 ∙ Irina Primac1 ∙ Frédéric Murisier1 ∙ Liliana Garcia-Martinez2 ∙ Rodrigo L. Borges2 ∙ Qingzeng Gao3,4 ∙ Pradeep K.R. Cingaram2 ∙ Emilie Calonne1 ∙ Bouchra Hassabi1 ∙ Céline Hubert1 ∙ Adèle Herpoel1 ∙ Pascale Putmans1 ∙ Frédérique Mies1 ∙ Jérôme Martin1 ∙ Louis [...]

Nature: Promotion of DNA end resection by BRCA1–BARD1 in homologous recombination (Sung, Libich)

September 12, 2024

 Sameer Salunkhe, James M. Daley, Hardeep Kaur, Nozomi Tomimatsu, Chaoyou Xue, Vivek B. Raina, Angela M. Jasper, Cody M. Rogers, Wenjing Li, Shuo Zhou, Rahul Mojidra, Youngho Kwon, Qingming Fang, Jae-Hoon Ji, Aida Badamchi Shabestari, O’Taveon Fitzgerald, Hoang Dinh, Bipasha Mukherjee, Amyn A. Habib, Robert Hromas, Alexander V. Mazin, Elizabeth V. Wasmuth, Shaun K. Olsen, [...]

Nature Communications: DSS1 restrains BRCA2’s engagement with dsDNA for homologous recombination, replication fork protection, and R-loop homeostasis (Zhao)

August 20, 2024

 Yuxin Huang, Wenjing Li, Tzeh Foo, Jae-Hoon Ji, Bo Wu, Nozomi Tomimatsu, Qingming Fang, Boya Gao, Melissa Long, Jingfei Xu, Rouf Maqbool, Bipasha Mukherjee, Tengyang Ni, Salvador Alejo, Yuan He, Sandeep Burma, Li Lan, Bing Xia & Weixing Zhao Abstract DSS1, essential for BRCA2-RAD51 dependent homologous recombination (HR), associates with the helical domain (HD) and OB-fold [...]

Science Advances: IRF8-mutant B cell lymphoma evades immunity through a CD74-dependent deregulation of antigen processing and presentation in MHCII complexes (Gupta Lab)

July 17, 2024

                   ZHIJUN QIU JIHANE KHALIFE,  PURUSHOTH ETHIRAJ CARINE JAAFAR,  AN-PING LIN,  KENNETH N. HOLDER,  JACOB P. RITTER,  LILLY CHIOU GABRIELA HUELGAS-MORALES SADIA ASLAM, ZHAO ZHANG ZHIJIE LIU SHAILEE ARYA,  YOGESH K. GUPTA PATRICIA L. M. DAHIA RICARDO C.T. AGUIAR Abstract The mechanism by which interferon regulatory factor 8 (IRF8) mutation contributes to [...]

Nucleic Acids Research: The protein phosphatase EYA4 promotes homologous recombination (HR) through dephosphorylation of tyrosine 315 on RAD51 (Sung Lab)

December 14, 2023

 Bárbara de la Peña Avalos, Nicolas Paquet, Romain Tropée, Yan Coulombe, Hannah Palacios, Justin W Leung, Jean-Yves Masson, Pascal H G Duijf, Eloïse Dray Abstract Efficient DNA repair and limitation of genome rearrangements rely on crosstalk between different DNA double-strand break (DSB) repair pathways and their synchronization with the cell cycle. The selection, timing, and efficacy of DSB repair pathways are influenced by post-translational modifications of histones and [...]

Molecular Cell: SRSF2 Plays an Unexpected Role as Reader of m5C on mRNA, Linking Epitranscriptomics to Cancer (Gupta)

December 7, 2023

 Hai-Li Ma 1 14, Martin Bizet 1 14, Christelle Soares Da Costa 1 14, Frédéric Murisier 1, Eric James de Bony 1 8, Meng-Ke Wang 2, Akihide Yoshimi 3 9, Kuan-Ting Lin 7, Kristin M. Riching 4, Xing Wang 2, John I. Beckman 5, Shailee Arya 5, Nathalie Droin 6, Emilie Calonne 1, Bouchra Hassabi 1, Qing-Yang Zhang 2 10, Ang Li 2 11, Pascale Putmans 1, Lionel Malbec 1, Céline Hubert 1…François Fuks 1 15 Highlights SRSF2 preferentially binds m5C-marked mRNA, whereas SRSF2P95H mutant impairs binding NSUN2 depletion reduces mRNA m5C levels and alters SRSF2 RNA binding and splicing NSUN2 loss and SRSF2P95H alter SRSF2 binding to key leukemia-related transcripts In leukemia patients, low NSUN2 levels and SRSF2P95H mutation predict poor outcomes. Summary A common mRNA [...]

Nucleic Acids Research: TATDN2 resolution of R-loops is required for survival of BRCA1-mutant cancer cells (Sung, Gupta, Pertsemlidis, Bishop, et al)

November 29, 2023

 Aruna S Jaiswal, Arijit Dutta, Gayathri Srinivasan, Yaxia Yuan, Daohong Zhou, Montaser Shaheen, Doraid T Sadideen, Austin Kirby, Elizabeth A Williamson, Yogesh K Gupta, Shaun K Olsen, Mingjiang Xu, Eva Loranc, Pramiti Mukhopadhyay, Alexander Pertsemlidis, Alexander J R Bishop, Patrick Sung, Jac A Nickoloff, Robert Hromas BRCA1-deficient cells have increased IRE1 RNase, which degrades multiple microRNAs. Reconstituting expression of one of these, miR-4638–5p, resulted in synthetic lethality in BRCA1-deficient cancer cells. We found that miR-4638-5p represses expression of [...]

Nucleic Acids Research: TATDN2 resolution of R-loops is required for survival of BRCA1-mutant cancer cells (Gupta, Pertsemlidis, Bishop, & Sung Labs)

November 20, 2023

 Aruna S Jaiswal, Arijit Dutta, Gayathri Srinivasan, Yaxia Yuan, Daohong Zhou, Montaser Shaheen, Doraid T Sadideen, Austin Kirby, Elizabeth A Williamson, Yogesh K Gupta, Shaun K Olsen, Mingjiang Xu, Eva Loranc, Pramiti Mukhopadhyay, Alexander Pertsemlidis, Alexander J R Bishop, Patrick Sung, Jac A Nickoloff, Robert Hromas Abstract BRCA1-deficient cells have increased IRE1 RNase, which degrades multiple microRNAs. Reconstituting expression of one of these, miR-4638–5p, resulted in synthetic lethality in BRCA1-deficient cancer cells. We found that miR-4638-5p represses [...]