Research Paper

Frontiers in Microbiology: Robust Heat Shock Response in Chlamydia Lacking a Typical Heat Shock Sigma Factor (Lai Lab)

January 20, 2022

Yehong Huang1,2†, Wurihan Wurihan2†, Bin Lu1,2†, Yi Zou3, Yuxuan Wang2†, Korri Weldon3, Joseph D. Fondell2†, Zhao Lai3,4, Xiang Wu1*†, and Huizhou Fan2*† Cells reprogram their transcriptome in response to stress, such as heat shock. In free-living bacteria, the transcriptomic reprogramming is mediated by increased DNA-binding activity of heat shock sigma factors and activation of genes normally repressed [...]

Frontiers in Genetics: Differential lncRNA/mRNA Expression Profiling and Functional Network Analyses in Bmp2 Deletion of Mouse Dental Papilla Cells (Lai Lab)

January 20, 2022

Feng Wang1,2†, Ran Tao1†, Li Zhao1, Xin-Hui Hao1, Yi Zou3, Qing Lin1, Meng Meng Liu2, Graham Goldman2, Daoshu Luo1* and Shuo Chen2* Bmp2 is essential for dentin development and formation. Bmp2 conditional knock-out (KO) mice display a similar tooth phenotype of dentinogenesis imperfecta (DGI). To elucidate a foundation for subsequent functional studies of cross-talk between [...]

iScience: SARS-CoV-2 infection enhances mitochondrial PTP complex activity to perturb cardiac energetics (Aune)

January 19, 2022

Karthik Ramachandran110 Soumya Maity110  Alagar R.Muthukumar2  Soundarya Kandala1  Dhanendra Tomar3  Tarek Mohamed Abd El-Aziz49  Cristel Allen1  Yuyang Sun5  Manigandan Venkatesan1  Travis  R.Madaris1  Kevin Chiem6  RachelTruitt7  Neelanjan Vishnu1  Gregory Aune8 Allen Anderson1 Luis Martinez6  Wenli Yang7  James D.Stockand3  Brij B.Singh5  Subramanya Srikantan1 W. BrianReeves1  Muniswamy Madesh111 Highlights • SARS-CoV-2 perturbs human ion channel, quality control, and [...]

Urologic Oncology: Prevalence of pathogenic germline cancer risk variants in testicular cancer patients: Identifying high risk groups (Tomlinson)

January 19, 2022

ChethanRamamurthyM.D.a‡Amin H.NassarM.D.bc‡SarahAbou AlaiwiM.D.bcElioAdibM.D.bcElie W.AklM.D.cShanYangPh.D.dEdward D.EsplinM.D., Ph.D.dMichael A.LissM.D.aGail E.TomlinsonM.D.eGuru P.SonpavdeM.D.b Highlights • Testicular cancer patients are at risk for carrying germline pathogenic variants. • Risk factors for germline variants include having another primary malignancy or a family history of testicular cancer. • A subset of patients with testicular cancer may particularly benefit from genetic evaluation. Abstract Background [...]

iScience: PEGylated talazoparib enhances therapeutic window of its combination with temozolomide in Ewing sarcoma (Kurmasheva & Houghton Labs)

January 18, 2022

Vanessa del Pozo Andrew J.Robles Shaun D.Fontaine QianqianLiu Joel E.Michalek Peter J.Houghton Raushan T.Kurmasheva Highlights Nanoparticle-formulated drugs minimize drug-induced toxicity PEG∼TLZ enhances in vivo activity of TMZ in pediatric tumor xenografts A 3-day interval between each drug's administration widens the therapeutic window A single IV dose of PEG∼TLZ is advantageous for treating infants/young children Summary [...]

Cellular Oncology: Single-cell RNA profiling identifies diverse cellular responses to EWSR1/FLI1 down regulation in Ewing sarcoma cells (Houghton, Chen, Kitagawa, Ignatius)

January 12, 2022

Roxane Khoogar, Fuyang Li, Yidong Chen, Myron Ignatius, Elizabeth R. Lawlor, Katsumi Kitagawa, Tim H.-M. Huang, Doris A. Phelps & Peter J. Houghton Background The EWSR1/FLI1 gene fusion is the most common rearrangement leading to cell transformation in Ewing sarcoma (ES). Previous studies have indicated that expression at the cellular level is heterogeneous and that levels [...]

Genes & Cancer: EZH2 suppresses endogenous retroviruses and an interferon response in cancers (Shiio Lab)

January 10, 2022

Panneerselvam Jayabal1, Xiuye Ma1 and Yuzuru Shiio1,2,3 ABSTRACT Ewing sarcoma is an aggressive cancer of the bone and soft tissue in children. It is characterized by the chromosomal translocation between EWS and an Ets family transcription factor, most commonly FLI1. We recently reported that Ewing sarcoma depends on the autocrine signaling mediated by a cytokine, NELL2. [...]

PMC: The Role of Hedgehog Pathway in Uterine Leiomyosarcoma (Houghton Lab)

January 7, 2022

Natalia Garcia,1 Mara Ulin,2 Ayman Al-Hendy,3 and Qiwei Yang3 DESCRIPTION The uterine Leiomyosarcoma (LMS) represents 3%-7% of all uterine cancers. This rare tumor has an annual incidence of 0.8 per 100,00 women. Unfortunately, LMS is well known for its poor prognosis due to its high recurrence rate and resistance to the currently available treatment options. These features open the [...]

MDPI: Altertoxin II, a Highly Effective and Specific Compound against Ewing Sarcoma (Houghton Lab)

January 6, 2022

Andrew J. Robles Wentao Dai Saikat Haldar Hongyan Ma Victoria M. Anderson Ross D. Overacker April L. Risinger, Sandra Loesgen Peter J. Houghton Robert H. Cichewicz Susan L. Mooberry Simple Summary Ewing sarcoma is a cancer of the bone and soft tissues that affects children and adolescents. Unfortunately, only 20–30% of patients with metastatic Ewing [...]

ASM Journals: GRWD1-WDR5-MLL2 Epigenetic Complex Mediates H3K4me3 Mark and Is Essential for Kaposi’s Sarcoma-Associated Herpesvirus-Induced Cellular Transformation (Lai)

January 5, 2022

Shan Wei, Songjian Lu, Lifan Liang, Xian Wang, Wan Li, Tingting Li, Luping Chen, , Enguo Ju, Xinquan Zhang, Zhao Lai, Yufei Huang, Xinghua Lu, Shou-Jiang Gao  ABSTRACT Infection by Kaposi’s sarcoma-associated herpesvirus (KSHV) is causally associated with numerous cancers. The mechanism of KSHV-induced oncogenesis remains unclear. By performing a CRISPR-Cas9 screening in a model of KSHV-induced cellular transformation of primary cells, we identified epigenetic regulators that were essential for KSHV-induced cellular transformation. Examination of TCGA data sets of the [...]