Journal of Biological Chemistry: The RHOX Homeodomain Proteins Regulate the Expression of Insulin and Other Metabolic Regulators in the Testis*

November 29, 2013

James A. MacLean II‡,§, Zhiying Hu§, Joshua P. Welborn‡, Hye-Won Song§,¶,‖, Manjeet K. Rao§,**, Chad M. Wayne§,‡‡ and Miles F. Wilkinson§,¶,‖1 Abstract Defects in cellular metabolism have been widely implicated in causing male infertility, but there has been little progress in understanding the underlying mechanism. Here we report that several key metabolism genes are regulated in […]

Bioinformatics: Exome-based analysis for RNA epigenome sequencing data

June 15, 2013

Jia Meng, Xiaodong Cui, Manjeet K. Rao, Yidong Chen, Yufei Huang Abstract Motivation: Fragmented RNA immunoprecipitation combined with RNA sequencing enabled the unbiased study of RNA epigenome at a near single-base resolution; however, unique features of this new type of data call for novel computational techniques. Result: Through examining the connections of RNA epigenome sequencing data with two well-studied data […]

PLOS One: Genomic Loss of Tumor Suppressor miRNA-204 Promotes Cancer Cell Migration and Invasion by Activating AKT/mTOR/Rac1 Signaling and Actin Reorganization

December 21, 2012

J. Saadi Imam, Jason R. Plyler , Hima Bansal , Suresh Prajapati , Sanjay Bansal, Jennifer Rebeles, Hung-I Harry Chen, Yao-Fu Chang, Subbarayalu Panneerdoss, Behyar Zoghi, Kalyan C. Buddavarapu, Russell Broaddus, Peter Hornsby, Gail Tomlinson, Jeffrey Dome, Ratna K. Vadlamudi, Alexander Pertsemlidis, Yidong Chen, Manjeet K. Rao  Abstract Increasing evidence suggests that chromosomal regions containing microRNAs are functionally important […]

Medical Express: New hope for taming triple-negative breast cancer

October 2, 2012

Disease-free survival is short-lived for women with triple-negative breast cancer—a form of the disease that doesn’t respond to hormone drugs and becomes resistant to chemotherapy. Thankfully, a promising line of study in the School of Medicine at The University of Texas Health Science Center San Antonio suggests it is possible to fine-tune the properties of […]

Journal of Biological Chemistry: The transcription factor Wilms tumor 1 confers resistance in myeloid leukemia cells against the proapoptotic therapeutic agent TRAIL (tumor necrosis factor α-related apoptosis-inducing ligand) by regulating the antiapoptotic protein Bcl-xL

September 21, 2012

Hima Bansal 1, Theresea Seifert, Carlos Bachier, Manjeet Rao, Gail Tomlinson, Swaminathan Padmanabhan Iyer, Sanjay Bansal Abstract Tumor necrosis factor α-related apoptosis-inducing ligand (TRAIL) is considered a promising cancer therapeutic agent due to its ability to induce apoptosis in a variety of cancer cells while sparing normal cells. However, many human tumors including acute myeloid leukemia (AML) are partially or completely resistant […]

PLOS One: Androgen-Responsive MicroRNAs in Mouse Sertoli Cells

July 20, 2012

Subbarayalu Panneerdoss, Yao-Fu Chang, Kalyan C. Buddavarapu, Hung-I Harry Chen, Gunapala Shetty, Huizhen Wang, Yidong Chen, T. Rajendra Kumar, Manjeet K. Rao  Abstract Although decades of research have established that androgen is essential for spermatogenesis, androgen’s mechanism of action remains elusive. This is in part because only a few androgen-responsive genes have been definitively identified in the testis. Here, we propose that […]

PNAS: Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation

April 10, 2012

Yao-Fu Chang, Jennifer S. Lee-Chang, J. Saadi Imam, Kalyan Chakravarthy Buddavarapu, Sarah S. Subaran, Amiya P. Sinha-Hikim, Myriam Gorospe, and Manjeet K. Rao Abstract Decoupling of transcription and translation during postmeiotic germ cell differentiation is critical for successful spermatogenesis. Here we establish that the interaction between microRNAs and actin-associated protein Arpc5 sets the stage for an elaborate translational control mechanism by facilitating […]

Biotechniques: Isolation of Sertoli, Leydig, and spermatogenic cells from the mouse testis

November 5, 2011

Yao-Fu Chang 1, Jennifer S Lee-Chang, Subbarayalu Panneerdoss, James A MacLean 2nd, Manjeet K Rao Abstract A thorough understanding of the events during mammalian spermatogenesis requires studying specific molecular signatures of individual testicular cell populations as well as their interaction in co-cultures. However, most purification techniques to isolate specific testicular cell populations are time-consuming, require large numbers of animals, and/or […]