Molecular Cell: SRSF2 Plays an Unexpected Role as Reader of m5C on mRNA, Linking Epitranscriptomics to Cancer (Gupta)

December 7, 2023

Hai-Li Ma 1 14, Martin Bizet 1 14, Christelle Soares Da Costa 1 14, Frédéric Murisier 1, Eric James de Bony 1 8, Meng-Ke Wang 2, Akihide Yoshimi 3 9, Kuan-Ting Lin 7, Kristin M. Riching 4, Xing Wang 2, John I. Beckman 5, Shailee Arya 5, Nathalie Droin 6, Emilie Calonne 1, Bouchra Hassabi 1, Qing-Yang Zhang 2 10, Ang Li 2 11, Pascale Putmans 1, Lionel Malbec 1, Céline Hubert 1…François Fuks 1 15 Highlights SRSF2 preferentially binds m5C-marked mRNA, whereas SRSF2P95H mutant impairs binding NSUN2 depletion reduces mRNA m5C levels and alters SRSF2 RNA binding and splicing NSUN2 loss and SRSF2P95H alter SRSF2 binding to key leukemia-related transcripts In leukemia patients, low NSUN2 levels and SRSF2P95H mutation predict poor outcomes. Summary A common mRNA [...]

Cold Spring Harbor, Spatial-Live: A lightweight and versatile tool for single cell spatial-omics data visualization (Chen, Zheng, Lai)

December 5, 2023

Zhenqing Ye, Zhao Lai, Siyuan Zheng, Yidong Chen Abstract Single-cell spatial-omics data visualization plays a pivotal role in unraveling the intricate spatial organization and heterogeneity of cellular systems. Although various software tools and packages have been developed for this purpose, challenges persist in terms of user-friendly accessibility, data integration, and interactivity. In this study, we introduce Spatial-Live, a lightweight and versatile viewer tool designed [...]

Molecular and Cellular Biology: A SNAI2/CTCF Interaction is Required for NOTCH1 Expression in Rhabdomyosarcoma (Ignatius Lab)

December 1, 2023

Prethish Sreenivas,Long Wang,Meng Wang,Anil Challa,Paulomi Modi,Nicole Rae Hensch,Berkley Gryder,Hsien-Chao Chou,Xiang R. Zhao,Benjamin Sunkel,Rodrigo Moreno-Campos,Javed Khan,Benjamin Z. Stanton & Myron S. Ignatius Abstract Rhabdomyosarcoma (RMS) is a pediatric malignancy of the muscle with characteristics of cells blocked in differentiation. NOTCH1 is an oncogene promoting self-renewal and blocking differentiation in the fusion negative-RMS sub-type. However, how NOTCH1 [...]

Nature Communications: Genomic profiling of subcutaneous patient-derived xenografts reveals immune constraints on tumor evolution in childhood solid cancer (Zheng, Grimes, Lai, Chen, Wang, Tomlinson, Houghton, & Kurmasheva Labs)

November 27, 2023

Funan He, Abhik M. Bandyopadhyay, Laura J. Klesse, Anna Rogojina, Sang H. Chun, Erin Butler, Taylor Hartshorne, Trevor Holland, Dawn Garcia, Korri Weldon, Luz-Nereida Perez Prado, Anne-Marie Langevin, Allison C. Grimes, Aaron Sugalski, Shafqat Shah, Chatchawin Assanasen, Zhao Lai, Yi Zou, Dias Kurmashev, Lin Xu, Yang Xie, Yidong Chen, Xiaojing Wang, Gail E. Tomlinson, Stephen [...]

Clinical Cancer Research: Surface and Global Proteome Analyses Identify ENPP1 and Other Surface Proteins as Actionable Immunotherapeutic Targets in Ewing Sarcoma (Kurmasheva)

October 18, 2023

Brian Mooney Gian Luca Negri Taras Shyp Alberto Delaidelli Hai-Feng Zhang Sandra E. Spencer Miko Amber K. Weiner Alexander B. Radaoui Rawan Shraim Michael M. Lizardo Christopher S. Hughes Amy Li Melanie Rouleau Wei Li Dimiter S. Dimitrov Raushan T. Kurmasheva Sharon J. Diskin John M. Maris Gregg B. Morin Poul H. Sorensen Abstract Purpose: [...]

Molecular Cell: DICER Ribonuclease Removes Harmful R-Loops (Sung)

October 18, 2023

Lola P. Camino 1 2 4, Arijit Dutta 3 4, Sonia Barroso 1 2, Carmen Pérez-Calero 1 2, Jeffrey N. Katz 3, María García-Rubio 1 2, Patrick Sung 3, Belén Gómez-González 1 2, Andrés Aguilera 1 2 5 Highlights •DICER RNase activity mediates R-loop processing in vitro and in cells •DICER binds R-loops readily but not DNA-RNA hybrids •DICER cleaves the RNA moiety of DNA-RNA hybrids within R-loop structures •DICER overexpression counteracts harmful nuclear DNA-RNA hybrids Summary R-loops, consisting of a DNA-RNA hybrid and a displaced DNA strand, threaten genome integrity. To counteract [...]

Cancers: Dysregulation of Type I Interferon (IFN-I) Signaling: A Potential Contributor to Racial Disparity in Hepatocellular Carcinoma (HCC) (Lai)

September 12, 2023

            Saranya Chidambaranathan Reghupaty 1,†,Sadia Kanwal 2, Rachel G. Mendoza 3, Eva Davis 4,Haiwen Li 2, Zhao Lai 5, Mikhail G. Dozmorov 6 ,Milton Omar Faison 7, Rafat Ali Siddiqui 2, *Devanand Sarkar 8,* Simple Summary Hepatocellular carcinoma (HCC) is the most common primary liver cancer arising from the liver [...]

Embro Reports: Loss of BRD4 induces cell senescence in HSC/HPCs by deregulating histone H3 clipping (Chen)

September 5, 2023

Abstract Bromodomain-containing protein 4 (BRD4) is overexpressed and functionally implicated in various myeloid malignancies. However, the role of BRD4 in normal hematopoiesis remains largely unknown. Here, utilizing an inducible Brd4 knockout mouse model, we find that deletion of Brd4 (Brd4Δ/Δ) in the hematopoietic system impairs hematopoietic stem cell (HSC) self-renewal and differentiation, which associates with cell cycle arrest and [...]

Science Reports: Sex-dependent differences in the genomic profile of lingual sensory neurons in naïve and tongue-tumor bearing mice (Chen, Lai)

August 14, 2023

Tarek Ibrahim, Ping Wu, Li-Ju Wang, Chang Fang-Mei, Josue Murillo, Jaclyn Merlo, Sergey S. Shein, Alexei V. Tumanov, Zhao Lai, Korri Weldon, Yidong Chen & Shivani Ruparel Abstract Mechanisms of sex-dependent orofacial pain are widely understudied. A significant gap in knowledge exists about the comprehensive regulation of tissue-specific trigeminal sensory neurons in diseased states of both [...]

Nucleic Acids Research: TLK1-Mediated RAD54 Phosphorylation Spatio-Temporally Regulates Homologous Recombination Repair (Chen, Sung)

July 13, 2023

Ishita Ghosh, Youngho Kwon, Aida Badamchi Shabestari, Rupesh Chikhale, Jing Chen, Claudia Wiese, Patrick Sung, Arrigo De Benedetti Abstract Environmental agents like ionizing radiation (IR) and chemotherapeutic drugs can cause severe damage to the DNA, often in the form of double-strand breaks (DSBs). Remaining unrepaired, DSBs can lead to chromosomal rearrangements and cell death. One major error-free pathway to repair DSBs is homologous [...]