Nature: EWS–FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma

March 7, 2018

Aparna Gorthi, July Carolina Romero, Eva Loranc, Lin Cao, Liesl A. Lawrence, Elicia Goodale, Amanda Balboni Iniguez, Xavier Bernard, V. Pragathi Masamsetti, Sydney Roston, Elizabeth R. Lawlor, Jeffrey A. Toretsky, Kimberly Stegmaier, Stephen L. Lessnick, Yidong Chen & Alexander J. R. Bishop Abstract Ewing sarcoma is an aggressive pediatric cancer of the bone and soft tissue. It results from a chromosomal translocation, predominantly t(11;22)(q24:q12), that fuses the N-terminal transactivation domain of […]

Molecular & Cellular Proteomics: Detection of proteome diversity resulted from alternative splicing is limited by trypsin cleavage specificity

March 1, 2018

Xiaojing Wang, Simona G Codreanu, Bo Wen, Kai Li, Matthew C Chambers, Daniel C Liebler, Bing Zhang Alternative splicing dramatically increases transcriptome complexity but its contribution to proteome diversity remains controversial. Exon-exon junction spanning peptides provide direct evidence for the translation of specific splice isoforms and are critical for delineating protein isoform complexity. Here we […]

Cell Stem Cell: Vangl2/RhoA Signaling Pathway Regulates Stem Cell Self-Renewal Programs and Growth in Rhabdomyosarcoma

March 1, 2018

Madeline N Hayes 1, Karin McCarthy 1, Alexander Jin 1, Mariana L Oliveira 2, Sowmya Iyer 1, Sara P Garcia 1, Sivasish Sindiri 3, Berkley Gryder 3, Zainab Motala 4, G Petur Nielsen 5, Jean-Paul Borg 6, Matt van de Rijn 7, David Malkin 4, Javed Khan 3, Myron S Ignatius 8, David M Langenau 9 Abstract Tumor growth and relapse are driven by tumor propagating cells (TPCs). However, mechanisms regulating TPC fate choices, maintenance, and self-renewal are not fully understood. Here, we show that Van […]

Cell: Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection

February 22, 2018

Author links open overlay panelShen-YingZhang12339, Nathaniel E.Clark436, Catherine A.Freije536, Elodie Pauwels136Allison J.Taggart636, SatoshiOkada737, Hanna Mandel8937, PaulaGarcia1037, Michael J.Ciancanelli137, Anat Biran137, Fabien G.Lafaille137, Miyuki Tsumura7, Aurélie Cobat23, Jingchuan Luo1112, StefanoV olpi13, Bastian Zimmer14Sonoko Sakata7Alexandra, Dinis15…Jean-Laurent Casanova1233435 Summary Viruses that are typically benign sometimes invade the brainstem in otherwise healthy children. We report bi-allelic DBR1 mutations in unrelated patients from different […]

European Urology: Germline DNA-repair Gene Mutations and Outcomes in Men with Metastatic Castration-resistant Prostate Cancer Receiving First-line Abiraterone and Enzalutamide

February 10, 2018

Emmanuel S.Antonarakisab†, ChangxueLub†  BrandonLubera  ChaoLiangb  HaoWanga  YanChenb  John L.Silbersteinb DaniloPianab ZhaoLaic YidongChencd William B.Isaacsab JunLuoab Abstract Background Inherited DNA-repair gene mutations are more prevalent in men with advanced prostate cancer than previously thought, but their clinical implications are not fully understood. Objective To investigate the clinical significance of germline DNA-repair gene alterations in men with metastatic castration-resistant prostate cancer (mCRPC) receiving next-generation hormonal […]

Cell Death & Disease: miR-195 targets cyclin D3 and survivin to modulate the tumorigenesis of non-small cell lung cancer

February 7, 2018

Xiaojie Yu, Yiqiang Zhang, David Cavazos, Xiuye Ma, Zhenze Zhao, Liqin Du & Alexander Pertsemlidis Abstract miR-195 has recently been reported to function as a tumor suppressor in various cancers, including non-small cell lung cancer (NSCLC). However, the mechanisms by which miR-195 represses the tumorigenesis of NSCLC cells are not fully understood. We performed a high-throughput […]

Childhood Obesity: Characterization of the Stool Microbiome in Hispanic Preschool Children by Weight Status and Time

February 1, 2018

Emily B Hollister 1 2, Byron A Foster 3, Mahmoud Dahdouli 1 2, Jesica Ramirez 4, Zhao Lai 5 Abstract Background: Variations in gut microbiota composition and diversity have been associated with childhood adiposity, although most studies describing this have been cross-sectional in nature. Our objective was to evaluate associations between body weight and the gut microbiota over time in obese preschool-age children. Methods: Obese, preschool-age, Hispanic children […]

Atlas of Science: The position of the centromere is marked with ubiquitylation of CENP-A

January 30, 2018

Chromosomes must segregate equally between daughter cells during cell division, and for proper chromosome segregation, spindle microtubules must attach to a single region of each chromosome, termed the “centromere,” in most eukaryotes. The kinetochore is a complex of proteins that is located at the centromere. Defects in this mechanism leads to chromosome instability (CIN). CIN, […]

Journal of Biological Chemistry: Cohesin SA2 is a sequence-independent DNA-binding protein that recognizes DNA replication and repair intermediates

January 19, 2018

Preston Countryman 1, Yanlin Fan 2, Aparna Gorthi 3 4, Hai Pan 1, Jack Strickland 1, Parminder Kaur 1, Xuechun Wang 5, Jiangguo Lin 1 6, Xiaoying Lei 2 7, Christian White 1, Changjiang You 8, Nicolas Wirth 9, Ingrid Tessmer 9, Jacob Piehler 8, Robert Riehn 1, Alexander J R Bishop 3 4, Yizhi Jane Tao 2, Hong Wang 10 11 PMID: 29175904 PMCID: PMC5777247 DOI: 10.1074/jbc.M117.806406 Abstract Proper chromosome alignment and segregation during mitosis depend on the cohesion between sister chromatids, mediated by the cohesin protein complex, which also plays crucial roles in diverse genome […]