Journal of the National Cancer Institute: Integrative multi-omics characterization of hepatocellular carcinoma in Hispanic patients (Zheng, Chen & Lai Labs)

September 4, 2024

Debodipta Das, PhD, Xiaojing Wang, PhD, Yu-Chiao Chiu, PhD, Hakim Bouamar, PhD, Francis E Sharkey, MD, Jorge E Lopera, MD, Zhao Lai, PhD, Susan T Weintraub, PhD, Xianlin Han, PhD, Yi Zou, MS, Hung-I H Chen, PhD, Carla R Zeballos Torrez, MD, PhD, Xiang Gu, PhD, Matyas Cserhati, PhD, Joel E Michalek, PhD, Glenn A Halff, MD, Yidong Chen, PhD, Siyuan Zheng, PhD, Francisco G Cigarroa, MD, Lu-Zhe Sun, Ph Abstract Background The [...]

eLife: SERBP1 interacts with PARP1 and is Present in PARylation-Dependent Protein Complexes Regulating Splicing, Cell Division, and Ribosome Biogenesis (Penalva, Libich, et al)

July 3, 2024

Kira Breunig Xiufen Lei Mauro Montalbano Gabriela D. A. Guardia Shiva Ostadrahimi Victoria Alers Adam Kosti Jennifer Chiou Nicole Klein Corina Vinarov Lily Wang Mujia Li Weidan Song W. Lee Kraus David S. Libich Stefano Tiziani Susan T. Weintraub Pedro A. F. Galante Luiz O. F. Penalva Abstract RNA binding proteins (RBPs) containing intrinsically disordered [...]

Journal of the American Chemical Society: ights into Molecular Diversity within the FUS/EWS/TAF15 Protein Family: Unraveling Phase Separation of the N-Terminal Low-Complexity Domain from RNA-Binding Protein EWS (Libich Lab)

April 8, 2024

Abstract The FET protein family, comprising FUS, EWS, and TAF15, plays crucial roles in mRNA maturation, transcriptional regulation, and DNA damage response. Clinically, they are linked to Ewing family tumors and neurodegenerative diseases such as amyotrophic lateral sclerosis. The fusion protein EWS::FLI1, the causative mutation of Ewing sarcoma, arises from a genomic translocation that fuses [...]

ELife: Defining function of wild-type and three patient specific TP53 mutations in a zebrafish model of embryonal rhabdomyosarcoma (Ignatius Lab, Houghton Lab, Libich Lab, Chen, Tomlinson)

June 2, 2023

Jiangfei Chen Kunal Baxi Amanda E Lipsitt Nicole Rae Hensch Long Wang Prethish Sreenivas Paulomi Modi Xiang Ru Zhao Antoine Baudin Daniel G Robledo Abhik Bandyopadhyay Aaron Sugalski Anil K Challa Dias Kurmashev Andrea R Gilbert Gail E Tomlinson Peter Houghton Yidong Chen Madeline N Hayes Eleanor Y Chen David S Libich Myron S Ignatius [...]

Biomolecules: Enhancing the Conformational Stability of the cl-Par-4 Tumor Suppressor via Site-Directed Mutagenesis (Libich Lab)

May 17, 2023

Samjhana Pandey Krishna K. Raut Andrea M. Clark  Antoine Baudin  Lamya Djemri  David S. Libich  Komala Ponniah Steven M. Pascal Abstract Intrinsically disordered proteins play important roles in cell signaling, and dysregulation of these proteins is associated with several diseases. Prostate apoptosis response-4 (Par-4), an approximately 40-kilodalton proapoptotic tumor suppressor, is a predominantly intrinsically disordered [...]

Biopolymers: Biochemical and biophysical characterization of the nucleic acid binding properties of the RNA/DNA binding protein EWS

March 21, 2023

Emily E. Selig,  Roohi Bhura,  Matthew R. White,  Shivani Akula,  Renee D. Hoffman,  Carmel N. Tovar,  Xiaoping Xu,  Rachell E. Booth,  David S. Libich Abstract EWS is a member of the FET family of RNA/DNA binding proteins that regulate crucial phases of nucleic acid metabolism. EWS comprises an N-terminal low-complexity domain (LCD) and a C-terminal RNA-binding domain (RBD). The RBD is further divided [...]

BioPhysics Journal: Unraveling the phase separation of EWS low complexity domain using molecular dynamics simulations (Libich Lab)

March 3, 2023

Kandarp A. Sojitra 1, Courtney Johnson 2 3, Erich Sohn 2 3, David Libich 2 3, Jeetain Mittal 1 EWS, an RNA-binding protein, is responsible for gene expression, cell signaling, RNA processing, and transport. It is a member of the FET (FUS, EWS, TAF15) family, whose derailed phase separation is known to cause many neurodegenerative [...]

Structure: Order and disorder bound together in SARS-CoV-2 Nsp1 suppress host translation (Libich Lab)

February 6, 2023

David S Libich 1, Antoine Baudin 2 Abstract In this issue of Structure, Wang et al. investigate the interplay between folded and disordered regions of the SARS-CoV-2 non-structural protein 1 (Nsp1) that promotes the suppression of host protein translation. Their investigation will lead to novel avenues to target critical viral functions necessary for host immune-response suppression therapeutically. Read [...]

Biomolecular NMR Assignments: The 1H, 15N and 13C resonance assignments of the low-complexity domain from the oncogenic fusion protein EWS-FLI1 (Libich Lab)

January 12, 2022

Courtney N. Johnson, Xiaoping Xu, Stephen P. Holloway & David S. Libich Abstract The RNA-binding protein EWS is a multifunctional protein with roles in the regulation of transcription and RNA splicing. It is one of the FET (FUS, EWS, and TAF15) family of RNA binding proteins that contain an intrinsically disordered, low-complexity N-terminal domain. The FET [...]

Childhood Cancer Q&A: David Libich, PhD

December 1, 2021

Asst Prof. David S. Libich, PhD,and members of his lab. What are some achievements you’ve made in advancing scientific knowledge relevant to childhood cancer research? My lab works on Ewing sarcoma, specifically on how the EWS-FLI1 oncogenic fusion protein misfunctions and promotes disease. We apply protein structural and biophysical approaches to understand how this protein [...]