DNA Repair Protocols: Identification of Genes Required for Damage Survival Using a Cell-Based RNAi Screen Against the Drosophila Genome

July 23, 2012

Abstract Exposure to DNA-damaging agents invokes biological responses necessary for damage recovery and cell survival. Despite the presence of intact DNA repair pathways, a lack of certain other biological pathways has been shown to sensitize cells to DNA-damaging agents’ exposure. It is likely that following DNA damage a complex interplay between DNA repair pathways and […]

PLOS One: Androgen-Responsive MicroRNAs in Mouse Sertoli Cells

July 20, 2012

Subbarayalu Panneerdoss, Yao-Fu Chang, Kalyan C. Buddavarapu, Hung-I Harry Chen, Gunapala Shetty, Huizhen Wang, Yidong Chen, T. Rajendra Kumar, Manjeet K. Rao  Abstract Although decades of research have established that androgen is essential for spermatogenesis, androgen’s mechanism of action remains elusive. This is in part because only a few androgen-responsive genes have been definitively identified in the testis. Here, we propose that […]

Pediatric Blood & Cancer: Genetics and epigenetics of hepatoblastoma

July 13, 2012

Gail E Tomlinson 1, Roland Kappler Abstract A number of unique genetic features are observed in hepatoblastoma that has provided insights into the origins of hepatoblastoma. Hallmark cytogenetic changes in hepatoblastoma include the acquisition of additional copies of whole chromosomes and a recurring unbalanced translocation involving 1q. Genetic syndromes are associated with approximately 15% of hepatoblastoma and […]

IEEE: Evaluating Dynamic Effects of Copy Number Alterations on Gene Expression Using a Single Transcription Model

July 13, 2012

Fang-Han Hsu; Erchin Serpedin; Yidong Chen; Edward R. Dougherty Abstract: DNA copy number alterations (CNAs) are known to be related to genetic diseases, including cancer. The unlimited transcription (UT) model, in which transcription occurs permissively with a simple activation probability, has been proposed to investigate the long-term effects of CNAs on gene expression values. The queueing theory was […]

Expert Opinion on Biological Therapy: microRNA regulation of cell viability and drug sensitivity in lung cancer

June 26, 2012

Liqin Du , & Alexander Pertsemlidis Abstract Introduction:microRNAs (miRNAs) are 19 – 23 nucleotide long RNAs found in multiple organisms that regulate gene expression and have been shown to play important roles in tumorigenesis. In the context of lung cancer, numerous studies have shown that tumor suppressor genes and oncogenes that play crucial roles in […]

Transnational Cancer Research: Chain-reaction, a model of gene expression dynamics

June 21, 2012

Submitted May 21, 2012. Accepted for publication Jun 21, 2012. doi: 10.3978/j.issn.2218-676X.2012.06.01 One of the fundamental studies of transcription response to stimuli is to model the activation, repression of hundreds of genes, and their regulations. To further complicate the scenario, genes are regulated by multiple transcription factors, operating through different modes (earlier activation or secondary […]

PLoS One: miR-337-3p and Its Targets STAT3 and RAP1A Modulate Taxane Sensitivity in Non-Small Cell Lung Cancers

June 18, 2012

Liqin Du, Maria C. Subauste, Christopher DeSevo, Zhenze Zhao, Michael Baker, Robert Borkowski, Jeoffrey J. Schageman, Rachel Greer, Chin-Rang Yang, Milind Suraokar, Ignacio I. Wistuba, Adi F. Gazdar, John D. Minna, Alexander Pertsemlidis  Abstract NSCLC (non-small cell lung cancer) often exhibits resistance to paclitaxel treatment. Identifying the elements regulating paclitaxel response will advance efforts to […]

Cancer Epidemiology Biomarkers & Prevention: Common genetic variation at BARD1 is not associated with breast cancer risk in BRCA1 or BRCA2 mutation carriers

May 11, 2012

Amanda B Spurdle 1, Louise Marquart, Lesley McGuffog, Sue Healey, Olga Sinilnikova, Fei Wan, Xiaoqing Chen, Jonathan Beesley, Christian F Singer, Anne-Catharine Dressler, Daphne Gschwantler-Kaulich, Joanne L Blum, Nadine Tung, Jeff Weitzel, Henry Lynch, Judy Garber, Douglas F Easton, Susan Peock, Margaret Cook, Clare T Oliver, Debra Frost, Don Conroy, D Gareth Evans, Fiona Lalloo, Ros Eeles, Louise Izatt, Rosemarie Davidson, Carol Chu, Diana Eccles, Christina G Selkirk, Mary Daly, Claudine Isaacs, Dominique Stoppa-Lyonnet, Olga M Sinilnikova, Bruno Buecher, Muriel Belotti, Sylvie Mazoyer, Laure Barjhoux, Carole Verny-Pierre, Christine Lasset, Hélène Dreyfus, Pascal Pujol, Marie-Agnès Collonge-Rame, GEMO Study Collaborators; Matti A […]

Science: Atg7 Modulates p53 Activity to Regulate Cell Cycle and Survival During Metabolic Stress

April 13, 2012

In Hye Lee1, Yoshichika Kawai1, Maria M. Fergusson1, Ilsa I. Rovira1, Alexander J. R. Bishop2, Noboru Motoyama3, Liu Cao4,*, Toren Finkel1,* Starvation and Autophagy Starvation stimulates withdrawal from the cell cycle, as well as stimulating autophagy. Are these two events connected? Lee et al. (p. 225) show a direct and nutrient-sensitive interaction between the tumor suppressor p53 and the essential autophagy gene […]

PNAS: Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation

April 10, 2012

Yao-Fu Chang, Jennifer S. Lee-Chang, J. Saadi Imam, Kalyan Chakravarthy Buddavarapu, Sarah S. Subaran, Amiya P. Sinha-Hikim, Myriam Gorospe, and Manjeet K. Rao Abstract Decoupling of transcription and translation during postmeiotic germ cell differentiation is critical for successful spermatogenesis. Here we establish that the interaction between microRNAs and actin-associated protein Arpc5 sets the stage for an elaborate translational control mechanism by facilitating […]