Molecular Cancer Therapeutics: Vertical Inhibition of the RAF-MEK-ERK Cascade Induces Myogenic Differentiation, Apoptosis and Tumor Regression in H/NRAS Q61X-mutant Rhabdomyosarcoma (Houghton, Chen, Ignatius, Vaseva)

November 8, 2021

 Natalia Garcia, Vanessa Del Pozo, Marielle E. Yohe, Craig M. Goodwin, Terry J Shackleford, Long Wang, Kunal Baxi, Yidong Chen, Anna T Rogojina, Sara M. Zimmerman, Cody J. Peer, William D. Figg, Myron S. Ignatius, Kris C Wood, Peter J. Houghton, and Angelina V Vaseva Abstract Oncogenic RAS signaling is an attractive target for fusion-negative rhabdomyosarcoma (FN-RMS). Our study validates the role of the ERK MAPK effector pathway in mediating RAS dependency in a panel of H/NRASQ61X-mutant RMS cells and correlates in vivo efficacy of the MEK [...]

Communications Biology: LIFR inhibition enhances the therapeutic efficacy of HDAC inhibitors in triple negative breast cancer (Chen)

November 1, 2021

 Mengxing Li, Suryavathi Viswanadhapalli, Bindu Santhamma, Uday P. Pratap, Yiliao Luo, Junhao Liu, Kristin A. Altwegg, Weiwei Tang, Zexuan Liu, Xiaonan Li, Behnam Ebrahimi, Hui Yan, Yi Zou, Swapna Konda, Gangadhara R. Sareddy, Zhenming Xu, Yidong Chen, Manjeet K. Rao, Andrew J. Brenner, Virginia G. Kaklamani, Rajeshwar R. Tekmal, Gulzar Ahmed, Ganesh V. Raj, Klaus [...]

Cancer Letters: KDM1A inhibition augments the efficacy of rapamycin for the treatment of endometrial cancer (Lai & Chen Labs)

October 28, 2021

 Prabhakar PittaVenkataa1 YihongChenab1 SalvadorAlejoa YiHeab Bridgitte E.Palaciosa IlannaLoeffela JunhaoLiuacUday P.Pratapa GabrielleGraya Sureshkumar MulampurathAchuthan Pillaia YiZoud ZhaoLaideT akayoshiSuzukif SuryavathiViswanadhapallia SrinathPalakurthig Rajeshwar R.Tekmalah Ratna K.Vadlamudiahi EdwardKosta Gangadhara R.Sareddyah Highlights • From the drug screen, we found that KDM1A inhibition is highly synergistic with mTOR inhibitors. • KDM1A inhibition attenuated the mTOR signaling cascade and abolished rapamycin-induced feedback [...]

Molecular Cancer Research: GLI3 Is Stabilized by SPOP Mutations and Promotes Castration Resistance via Functional Cooperation with Androgen Receptor in Prostate Cancer (Chen)

October 27, 2021

 Marieke Burleson, Janice J. Deng, Tai Qin, Thu Minh Duong, Yuqian Yan, Xiang Gu, Debodipta Das, Acarizia Easley, Michael A. Liss, P. Renee Yew, Roble Bedolla, Addanki Pratap Kumar, Tim Hui-Ming Huang, Yi Zou, Yidong Chen, Chun-Liang Chen, Haojie Huang, Lu-Zhe Sun and Thomas G. Boyer Abstract Although the Sonic hedgehog (SHH) signaling pathway has been implicated in promoting malignant phenotypes of prostate cancer, details on how it is activated and exerts its oncogenic role during prostate cancer development and progression are less clear. Here, we show that GLI3, a key SHH pathway [...]

Free Radical Biology and Medicine: Thioredoxin reductase-1 levels are associated with NRF2 pathway activation and tumor recurrence in non-small cell lung cancer (Bishop Lab)

October 26, 2021

 MarinaDelgoboa  Rosângela MayerGonçalvesag  Marco AntônioDelazerib  MarceloFalchettia  AlessandroZandonáb  RaquelNascimento das Nevesa  KarolineAlmeidaa  Adriane CristinaFagundesa  Daniel PensGelainc  João IsidroFracassod  Guilherme Baroni deMacêdod  LeonardoPriorid  NicklasBassanie  Alexander James RoyBishopef  Cassiano MateusForcelinib  José Cláudio FonsecaMoreirac1  AlfeuZanotto-Filhoa1 Highlights • Amid 64 redox genes, TXNRD1 shows association with poor survival in NSCLC. • High TXNRD1 levels predict poor DFS and DMFS in [...]

JoVE: Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins (Libich Lab)

October 13, 2021

 Courtney N. Johnson1, David S. Libich1 Abstract Intrinsically disordered proteins and intrinsically disordered regions within proteins make up a large and functionally significant part of the human proteome. The highly flexible nature of these sequences allows them to form weak, long-range, and transient interactions with diverse biomolecular partners. Specific yet low-affinity interactions promote promiscuous binding [...]

Frontiers in Molecular BioScience: Structural Characterization of the RNA-Binding Protein SERBP1 Reveals Intrinsic Disorder and Atypical RNA Binding Modes (Libich & Penalva labs)

October 13, 2021

 Antoine Baudin, 1 , 2 , † Alma K. Moreno-Romero, 1 , 2 Xiaoping Xu, 1 , 2 Emily E. Selig, 1 , 2 , † Luiz O. F. Penalva, 1 , 3 and David S. Libich 1 , 2 ,* † Abstract RNA binding proteins (RBPs) are essential for critical biological processes such as translation regulation and mRNA processing, and misfunctions of these proteins are associated with diseases such as cancer and neurodegeneration. SERBP1 (SERPINE1 [...]

Journal of Virology: MULTIPLEXED SIV-SPECIFIC PAIRED RNA-GUIDED CAS9 NICKASES INACTIVATE PROVIRAL DNA (Lai)

September 23, 2021

Authors: Lisa M Smith, Jason T. Ladner, Vida L. Hodara, Laura M. Parodi, R. Alan Harris, Jessica E. Callery, Zhao Lai, Yi Zou, Muthuswamy Raveedran, Jeffrey Rogers, and Luis D. Giavedoni Abstract Human and simian immunodeficiency virus infections establish a lifelong reservoir of cells harboring an integrated proviral genome. Genome editing CRISPR-associated Cas9 nucleases, combined […]