American Journal of Surgery: Polytrauma independent of therapeutic intervention alters the gastrointestinal microbiome

October 1, 2018

Susannah E Nicholson 1, Daniel Merrill 2, Caroline Zhu 3, David M Burmeister 4, Yi Zou 5, Zhao Lai 6, Daniel N Darlington 7, Aaron M Lewis 8, Larry Newton 9, Shannon Scroggins 10, Brian J Eastridge 11, Martin G Schwacha 12 Abstract Background: This study characterizes the gastrointestinal (GI) microbiome in a pre-clinical polytrauma hemorrhage model. Methods: Rats (n = 6) were anesthetized, hemorrhaged 20% of their blood volume, and subjected to a femur fracture and crush injuries to […]

Oncology: Immunogenic Heterogeneity of Renal Cell Carcinoma With Venous Tumor Thrombus

September 18, 2018

Author links open overlay panelMichael A.Lissabcd  YidongChenabcd  RonaldRodriguezabcd  DeepakPruthiabcd  TeresaJohnson-PaisabcdHanzhangWangabcd  AhmedMansourabcd  DharamKaushikabcd Abstract Objective To perform immune-cell enumeration and programmed death-ligand 1 (PD-L1) expression in clear cell renal cell carcinoma (cc-RCC) with tumor thrombus (TT) to guide therapeutic decisions. Methods After obtaining IRB approval and surgical consent, 6 patients underwent radical nephrectomy with venous tumor thrombectomy. We utilized RNA Sequencing to obtain RNAseq expression profiles. Computational […]

Elife: tp53 deficiency causes a wide tumor spectrum and increases embryonal rhabdomyosarcoma metastasis in zebrafish

September 7, 2018

Myron S Ignatius # 1 2 3 4, Madeline N Hayes # 1 2 3, Finola E Moore 1 2 3, Qin Tang 1 2 3, Sara P Garcia 1, Patrick R Blackburn 5, Kunal Baxi 4, Long Wang 4, Alexander Jin 1, Ashwin Ramakrishnan 1, Sophia Reeder 1, Yidong Chen 4, Gunnlaugur Petur Nielsen 1 2, Eleanor Y Chen 6, Robert P Hasserjian 1 2, Franck Tirode 7, Stephen C Ekker 8, David M Langenau 1 2 3 Abstract The TP53 tumor-suppressor gene is mutated in >50% of human tumors and Li-Fraumeni patients with germline inactivation are predisposed to developing cancer. Here, we generated tp53 deleted […]

eLife: tp53 deficiency causes a wide tumor spectrum and increases embryonal rhabdomyosarcoma metastasis in zebrafish

September 7, 2018

Myron S Ignatius, Madeline N Hayes, Finola E Moore, Qin Tang, Sara P Garcia, Patrick R Blackburn, Kunal Baxi, Long Wang, Alexander Jin, Ashwin Ramakrishnan, Sophia Reeder, Yidong Chen, Gunnlaugur Petur Nielsen, Eleanor Y Chen, Robert P Hasserjian, Franck Tirode, Stephen C Ekker, David M Langenau  Abstract The TP53 tumor-suppressor gene is mutated in >50% of human tumors and Li-Fraumeni patients with germline inactivation are predisposed to developing cancer. Here, we generated tp53 deleted […]

JCI Insight: IL-6 and CXCL8 mediate osteosarcoma-lung interactions critical to metastasis

August 23, 2018

 Amy C. Gross,1 Hakan Cam,1,2 Doris A. Phelps,3 Amanda J. Saraf,2,4 Hemant K. Bid,1,2 Maren Cam,1 Cheryl A. London,5,6 Sarah A. Winget,1 Michael A. Arnold,7,8 Laura Brandolini,9 Xiaokui Mo,10 John M. Hinckley,1,11 Peter J. Houghton,3 and Ryan D. Roberts1,2,4 Abstract: Osteosarcoma (OS), a malignant tumor of bone, kills through aggressive metastatic spread almost exclusively to the lung. Mechanisms driving this tropism for lung tissue remain unknown, though likely invoke [...]

CRISPR – Journal: CRISPR-Based Editing Reveals Edge-Specific Effects in Biological Networks

August 1, 2018

 Yi Li, Chance M. Nowak, Daniel Withers, Alexander Pertsemlidis, Leonidas Bleris Abstract Unraveling the properties of biological networks is central to understanding both normal and disease cellular phenotypes. Networks consist of functional elements (nodes) that form a variety of diverse connections (edges), with each node being a hub for multiple edges. Herein, in contrast to node-centric network perturbation [...]

Cancer Letters: miR-195 potentiates the efficacy of microtubule-targeting agents in non-small cell lung cancer

July 28, 2018

Xiaojie Yuab, Yiqiang Zhanga, Xiuye Maa, Alexander Pertsemlidisabc Abstract Microtubule-targeting agents (MTAs) are widely used for the treatment of non-small cell lung cancer (NSCLC). The response rate is only ∼25%, mainly attributable to drug resistance. To identify the determinants of resistance in NSCLC, we performed a high-throughput screen using a library of miRNA mimics. Here we report that […]

Oncotarget: Increased expression of the thyroid hormone nuclear receptor TRα1 characterizes intestinal tumors with high Wnt activity

July 24, 2018

Joel Uchuya-Castillo, Nicolas Aznar, Carla Frau, Pierre Martinez, Clementine Le Nevé, Laetitia Marisa, Luiz OF Penalva, Pierre Laurent-Puig, Alain Puisieux, Jean-Yves Scoazec, Jacques Samarut, Stephane Ansieau, Michelina Plateroti Our previous work demonstrated a key function of the thyroid hormone nuclear receptor TRα1, a T3-modulated transcription factor, in controlling intestinal development and homeostasis via the Wnt […]

Journal of Clinical Oncology: Cardiovascular Disease in Survivors of Childhood Cancer: Insights Into Epidemiology, Pathophysiology, and Prevention

July 20, 2018

Saro H. Armenian, Gregory T. Armstrong, Gregory Aune, Eric J. Chow, Matthew J. Ehrhardt, Bonnie Ky,  ABSTRACT Cardiovascular disease (CVD), which includes cardiomyopathy/heart failure, coronary artery disease, stroke, pericardial disease, arrhythmias, and valvular and vascular dysfunction, is a major concern for long-term survivors of childhood cancer. There is clear evidence of increased risk of CVD largely attributable to treatment exposures […]